Stagliano, who was a Chippendales dancer before entering porn, is best known for his Buttman series of films. Stagliano has stated that a scene showing Tracey Adams' buttocks inspired him to make the Buttman series of films.
In 1997, Stagliano tested positive for HIV. To date, medication has kept the virus in check. He continues to produce adult films and still acts in non-sexual roles. He married former porn star Tricia Devereaux.
Stagliano owns the adult video production company Evil Angel. The company distributes porn from directors such as Rocco Siffredi, Christoph Clark, John Leslie, Joey Silvera and Belladonna.
From October 2004 through February 2008, Stagliano produced and directed a Las Vegas show called Fashionistas based on his porn film of the same name.
On April 8, 2008 it was reported on the adult website XBIZ that John Stagliano had been charged with 8 obscenity Violations. On April 14, 2008, Stagliano launched a website, DefendOurPorn.org to post articles, news, and updates regarding the court proceedings.